- Ian Hunter
- Theseus - Duke of Athens
- Verree Teasdale
- Hippolyta - Queen of the Amazons - Betrothed to Theseus
- Hobart Cavanaugh
- Philostrate - Master of Revels to Theseus
- Dick Powell
- Lysander - In Love with Hermia
- Ross Alexander
- Demetrius - In Love with Hermia
- Olivia de Havilland
- Hermia - In Love with Lysander
- Jean Muir
- Helena - In Love with Demetrius
- Grant Mitchell
- Egeus - Father to Hermia
- Frank McHugh
- Quince - the Carpenter
- Dewey Robinson
- Snug - the Joiner
- James Cagney
- Bottom - the Weaver
- Joe E. Brown
- Flute - the Bellows-Mender
- Hugh Herbert
- Snout - the Tinker
- Otis Harlan
- Starveling - the Tailor
- Arthur Treacher
- Epilogue
- Victor Jory
- Oberon - King of the Fairies
- Anita Louise
- Titania - Queen of the Fairies
- Nini Theilade
- Fairie - Attending Titania
- Mickey Rooney
- Puck or Robin Goodfellow - a Fairy
- Kathy Frye
- Pease-Blossom / Fairie (as Kathryn Frye)
- Helen Westcott
- Cobweb
- Fred Sale Jr.
- Moth
- Billy Barty
- Mustard-Seed
- Baby Charlene Barry
- Fairy
- Eleanor Bayley
- Lady of the Court
- Shirley Bloomfield
- Fairy
- Sheila Brown
- Changeling Prince
- Jackie Butler
- Child
- Carrie Daumery
- Performance Audience Member
- Ruth Eddings
- Lady of the Court
- Beverly Edgecomb
- Fairy
- Carol Ellis
- Titania
- Jimmy Fay
- Fairy
- Patsy Galloway
- Attendant to Titania
- Shep Houghton
- Dancer
- Frances Hughes
- Fairy
- Lucyle Keeling
- Lady of the Court
- Margaret Kerry
- Fairy
- Joan Lott
- Attendant to Titania
- Bonnie Lynn
- Fairy
- Diana Mack
- Lady of the Court
- Roger McGee
- Fairy
- Martha Merrill
- Lady of the Court
- Joyce Oliver
- Fairy
- Rags Ragland
- Acting Troupe Member
- Angelo Rossitto
- Gnome
- Lorraine Rugg
- Fairy
- Betty June Sissom
- Fairy
- Marilyn Spinner
- Fairy
- Donn Stokes
- Child
- Shirley Van
- Fairy / Dancer (uncredited)
- Victoria Vinton
- Lady of the Court
- Helen Wood
- Lady of the Court
