Diego Luna Alexander was born on December 29, 1979 in Mexico City, Mexico, to Alejandro Luna and Fiona Alexander, who worked as a costume designer. His father is Mexican and his mother was British, of Scottish and English descent. His mother died in a car accident when Diego was only two. He soon became immersed in his father's passion, entertainment - Alejandro is the most acclaimed living theatre, cinema, and opera set designer in Mexico.

From an early age he began acting working in TV, movies, and theater. His first television role was in the movie El último fin de año (1991). His next role was in the Mexican soap opera El abuelo y yo (1992). His childhood best friend and fellow actor Gael Garcia Bernal played the title role. After 'El Abuelo y Yo', Diego began to receive more and more parts in theater, movies, and TV. His big break came in 2001 when he was cast in the critically-acclaimed Y tu mamá también (2001), once again alongside his best friend Gael García Bernal, as Tenoch Iturbide.

His star continues to shine and he is making a name for himself in the American market such as starring alongside Bon Jovi in Vampires: Los Muertos (2002) and the Oscar-winning Frida (2002).

In 2004, he starred in 'Havana Nights: Dirty Dancing 2', the prequel to 'Dirty Dancing', and is working on more projects in both Latin America and the United States.