A native of Toronto, Canada, Jessica Steen is probably best known in the U.S. for having been a regular on two short-lived TV series: the critically acclaimed Homefront (1991) and the science fiction show Earth 2 (1994). She also appeared opposite Michael Richards (Kramer from Seinfeld (1989)) in the film Trial and Error (1997). She was additionally the only female regular on the 1987 syndicated show, Captain Power and the Soldiers of the Future (1987), and has appeared as a guest star on a number of other TV series including ER (1994), Touched by an Angel (1994), Murder One (1995), and The Outer Limits (1995).

Steen was later cast as Constable Donna Sabine in the acclaimed Canadian police series Flashpoint (2008), and Special Agent Paula Cassidy in the popular CBS drama NCIS: Naval Criminal Investigative Service (2003). In 2012 she appeared as Commissioner Eva Braden in the dark comedy miniseries Bullet in the Face (2012), written by Alan Spencer. She has done several Lifetime and Hallmark TV movies to date, and has also made an appearance in the Facebook Watch TV series Sacred Lies (2018) (2020). Steen landed the role of Lisa Stillman, which was initially intended to be guest stint, on Heartland (2007), which is the longest-running hour-long scripted drama in Canadian TV history.