Robert Horton was born on July 29, 1924 into a Mormon family in Los Angeles, California as Meade Howard Horton Jr. He began his contract career at MGM in 1952 and adopted the acting name of Robert Horton.

Following his final role (as a guest star on Murder, She Wrote (1984)), Horton retired from acting in 1989. He had appeared in films, musical theatre, and many television series in both starring and guest roles, including Apache War Smoke (1952), Alfred Hitchcock Presents (1955), Wagon Train (1957), The Barbara Stanwyck Show (1960), Matinee Theater (1955), As the World Turns (1956), and The Green Slime (1968).

Horton was thrice married: to Mary Catherine Jobe, to Barbara Ruick, and to Marilynn Bradley, who survived him. He died on March 9, 2016, aged 91, in his native Los Angeles.