Will Yun Lee was born in Arlington, Virginia, to Jung Ja Lee and Soo Won Lee, who had recently emigrated from Korea. He was raised by both immediate and extended family and moved often, exposed to life on the tough Bronx streets and idyllic Hawaiian beaches. By his teens he was living in the San Francisco area with his father, a Korean Tae Kwon Do Grandmaster. Lee also became an accomplished martial artist and won an athletic scholarship to the University of California at Berkeley.

While in school, Lee worked at the East Bay Asian Youth Centre teaching high-risk teens from ghetto neighborhoods not unlike those he had known as a child in the Bronx. It was there that his ongoing commitment to young people began, a commitment that continues to play an important role in his life.

It was also at this time that he became seriously interested in acting, and after landing a role in Nash Bridges he moved to Los Angeles to pursue his career. Guest star roles in series such as "Profiler" and "Brimstone" led to a role in the TV movie The Disciples for UPN, and soon after to "What's Cooking" and TNT's "Witchblade".

In 2002 he was named by People as one of their "50 Most Beautiful People" which quickly lead to high profile roles in "Die Another Day", "Torque" and "Elektra". He has also acted on FX Network's television series "Thief", ABC Family's "Fallen" and was one of the main characters of NBC's science fiction television drama "Bionic Woman".

In November 2007, he was again recognized by People, this time as one of the members of their list of the 15 "Sexiest Men Alive". Most recently he appeared as Sang Min in the pilot of the hit CBS series "Hawaii Five O".

In August 2010, Will Yun Lee starred alongside Miguel Ferrer in the indie thriller "Far Away Eyes", which was shot entirely on location in Hong Kong.