Kohl Sudduth was born on August 8, 1974, in Granada Hills, California, and grew up in Circleville, Ohio. He earned a B.A. in English at Ohio University before moving to New York City at age 21 to pursue an acting career. After gaining experience in a handful of films, he landed a supporting role in Bowfinger (1999) starring Steve Martin and Eddie Murphy. He then went on to play a series regular in Darren Star's critically acclaimed Grosse Pointe (2000).

Brother of Third Watch (1999) star Skipp Sudduth, he has appeared regularly on stage in New York, including Richard Greenberg's multiple Tony Award-winning "Take Me Out" and "The Last Days of Judas Iscariot" starring Sam Rockwell and directed by Philip Seymour Hoffman.