- David Kaye
 
                    -  Optimus Prime / ... 42 episodes, 2007-2009
 
                
                
                
                    
- Jeff Bennett
 
                    -  Prowl / ... 39 episodes, 2007-2009
 
                
                
                
                    
- Bumper Robinson
 
                    -  Bumblebee / ... 39 episodes, 2007-2009
 
                
                
                
                    
- Tom Kenny
 
                    -  Isaac Sumdac / ... 36 episodes, 2007-2009
 
                
                
                
                    
- Bill Fagerbakke
 
                    -  Bulkhead / ... 36 episodes, 2007-2009
 
                
                
                
                    
- Corey Burton
 
                    -  Ratchet / ... 35 episodes, 2007-2009
 
                
                
                
                    
- Tara Strong
 
                    -  Sari Sumdac / ... 34 episodes, 2007-2009
 
                
                
                
                    
- Townsend Coleman
 
                    -  Sentinel Prime / ... 13 episodes, 2007-2009
 
                
                
                
                    
- Phil LaMarr
 
                    -  Jazz / ... 12 episodes, 2008-2009
 
                
                
                
                    
- Cree Summer
 
                    -  Blackarachnia / ... 6 episodes, 2007-2009
 
                
                
                
                    
- Alexander Polinsky
 
                    -  Headmaster / ... 4 episodes, 2008-2009
 
                
                
                
                    
- Susan Blu
 
                    -  Arcee / ... 4 episodes, 2008-2009
 
                
                
                
                    
- John Moschitta Jr.
 
                    -  Blurr 4 episodes, 2008-2009
 
                
                
                
                    
- Lance Henriksen
 
                    -  Lockdown 3 episodes, 2008-2009
 
                
                
                
                    
- Peter Stormare
 
                    -  Meltdown / ... 3 episodes, 2008
 
                
                
                
                    
- Kath Soucie
 
                    -  Professor Princess / ... 2 episodes, 2008
 
                
                
                
                    
- Fred Willard
 
                    -  Swindle 2 episodes, 2008-2009
 
                
                
                
                    
- 'Weird Al' Yankovic
 
                    -  Wreck-Gar / ... 2 episodes, 2008-2009
 
                
                
                
                    
- Brian Posehn
 
                    -  Nanosec / ... 2 episodes, 2008
 
                
                
                
                    
- Kevin Michael Richardson
 
                    -  Omega Supreme 1 episode, 2008
 
                
                
                
                    
- Judd Nelson
 
                    -  Rodimus Prime 1 episode, 2009
 
                
                
                
                    
- George Takei
 
                    -  Yoketron 1 episode, 2009
 
                
                
                
                    
- John Mariano
 
                    -  Dirt Boss 1 episode, 2009