- Rex Lease
- Kit Cardigan / John C. Cardigan [Ch. 1]
- Lona Andre
- Belle Meade
- William Farnum
- James Fitzpatrick
- Ruth Mix
- Elizabeth Custer [Chs. 1,3, 8-11,15]
- Jack Mulhall
- Lieutenant Cook
- Nancy Caswell
- Barbara Trent
- George Chesebro
- Lieutenant Frank Roberts
- Dorothy Gulliver
- Red Fawn
- Frank McGlynn Jr.
- General George A. Custer
- Helen Gibson
- Calamity Jane [Chs. 4-7, 10]
- Josef Swickard
- Major Henry Trent MD
- Chief Thundercloud
- Young Wolf
- Reed Howes
- Tom 'Keen' Blade
- Bobby Nelson
- Bobby Nelson
- Robert Walker
- Pete - Henchman
- Marty Joyce
- Buzz
- Milburn Morante
- Buckskin
- George Morrell
- Sgt.-Maj. Flannigan
- Creighton Hale
- Hank
- Ted Adams
- Barney / Buffalo Bill Cody [Chs. 6-7]
- Carl Mathews
- Chief True Eagle [Chs.1, 15] / Curly - Custer's orderly
- Allen Greer
- Wild Bill Hickok [Chs.7-10]
- High Eagle
- Chief Crazy Horse
- Howling Wolf
- Chief Sitting Bull
- Chief John Big Tree
- Medicine Man [Ch. 9]
- Iron Eyes Cody
- Chief Brown Fox
- Chief Thunderbird
- Rain-in-Face
- William Desmond
- Wagon Master [Ch. 1]
- Walter James
- Judge Hooker [Chs.12-14]
- James Sheridan
- Henchman Jim
- Ken Cooper
- Henchman - Ch. 5
- Budd Buster
- Major Ware [Chs. 8-9]
- Carter Wayne
- Striker Martin - Orderly
- Cactus Mack
- Lieutenant Weir [Chs. 10 & 15]
- Barney Furey
- Sgt.-Maj. Peters
- Lafe McKee
- Captain Benteen [Ch. 14]
- Franklyn Farnum
- Maj. Marcus Reno [Chs. 11, 14, 15]
- Ed Withrow
- Blue Crow [Chs. 10-11]
- Artie Ortego
- Indian [Ch. 5] / Quirk [Chs. 7, 13]
- William Bartlett
- Renegade
- Humming Bird
- Renegade
- Fred Burns
- Wagon Driver
- Yakima Canutt
- Renegade / Indian (uncredited)
- J.W. Cody
- Indian
- Little Eagle
- Renegade
- Buddy Fisher
- Renegade
- Walter Gable
- Renegade
- Gaston Glass
- Joe - Bartender
- Herman Hack
- Henchman
- William Hunt
- Renegade
- Charles Hunter
- Renegade
- Herb Jackson
- Renegade
- Wilbur McGaugh
- Man Who Brings Gun [Ch. 12]
- Lone Pine
- Indian
- Potter Poe
- Crow Scout
- Whitey Sovern
- Renegade
- Red Star
- Indian
- Mabel Strickland
- Mabel - Pioneer Woman
- Swift Eagle
- Indian
- Bill Thompson
- Renegade
- Tall Tree
- Renegade
- Chief White Feather
- Renegade
